Electroporation-Based Technologies and Treatments (EBTT)
Postgraduate Course and Scientific Workshop
Ljubljana, November 13—20 2016.
10th Anniversary


15th of September, 2016
International Society for Electroporation-Based Technologies and Treatments
- President:                                
Damijan Miklavčič
- Officer: Biological sciences
Marie-Pierre Rols



Recent Publications


Co-authored papers between French and Sloven partners


Casciola M, Kasimova MA, Rems L, Zullino S, Apollonio F, Tarek M. Properties of lipid electropores I: Molecular dynamics simulations of stabilized pores by constant charge imbalance. Bioelectrochemistry 109: 108-116, 2016.

Pillet F, Marjanovič I, Reberšek M, Miklavčič D, Rols MP, Kotnik T. Inactivation of spores by electric arcs. BMC Microbiol. 16: 148, 2016.

Raso J, Frey W, Ferrari G, Pataro G, Knorr D, Teissié J, Miklavčič D. Recommendations guidelines on the key information to be reported in studies of application of PEF technology in food and biotechnological processes. Innov. Food Sci. Emerg. Technol. 37: 312-321, 2016.

Rems L, Tarek M, Casciola M, Miklavčič D. Properties of lipid electropores II: Comparison of continuum-level modeling of pore conductance to molecular dynamics simulations. Bioelectrochemistry 112: 112-124, 2016.

Rosazza C, Haberl Meglič S, Zumbusch A, Rols MP, Miklavčič D. Gene electrotransfer: a mechanistic perspective. Curr. Gene Ther. 16: 98-129, 2016.

Miklavcic, L.M. Mir and P. T. Vernier. Foreword to Sixth Special Issue on Electroporation-Based Technologies and Treatments. J Membrane Biol 249:591, 2016

Tozon N, Lampreht Tratar U, Žnidar K, Sersa G, Teissié J, Čemazar M. Operating procedures of the electrochemotherapy for treatment of tumor in dogs and cats. Journal of visualized experiments, ISSN 1940-087X, Oct. 2016, iss. 116. http://www.jove.com/video/54760/operating-procedures-electrochemotherapy-for-treatment-tumor-dogs, doi: 10.3791/54760.

Kamensek U, Rols M-P, Čemazar M, Golzio M. Visualization of nonspecific antitumor effectiveness and vascular effects of gene electro-transfer to tumors. Current gene therapy, 2016, vol. 16, 90-97.



Papers published within the scope of the LEA


Silve, I. Leray, C. Poignard and L M. Mir. Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses. Scientific Reports, Volume: 6, Article Number: 19957, Published: FEB 1 2016

L.L. Lesueur, L.M. Mir and F.M. André. Overcoming the specific toxicity of large plasmids electrotransfer in primary cells in vitro. Molecular Therapy - Nucleic Acids, Vol. 5, article number e291, Published March 8 2016; doi:10.1038/mtna.2016.4

Calvet and L. M. Mir. The promising alliance of anticancer electrochemotherapy with immunotherapy. Cancer Metastases Reviews, 35(2), 165-177, 2016 DOI: 10.1007/s10555-016-9615-3

M.-A. de Menorval, F. Andre, A. Silve, C. Dalmay, O. Français, B. Le Pioufle, and L. M. Mir. Electric pulses: a flexible tool to manipulate cytosolic calcium concentrations and generate spontaneous-like calcium oscillations in mesenchymal stem cells. Sci. Rep. 6, 32331; doi: 10.1038/srep32331 (2016).

Tamošiūnas, L.M. Mir, W-S. Chen, A. Lihachev, M. Venslauskas and S. Šatkauskas. Intracellular delivery of bleomycin by combined application of electroporation and sonoporation in vitro. J Membrane Biol, 249: 677-700, 2016.

Denzi, E. della Valle, F. Apollonio, M. Breton, L. M. Mir and M. Liberti. Exploring the Applicability of Nano-poration for Remote Control in Smart Drug Delivery Systems. J Membrane Biol, DOI 10.1007/s00232-016-9922-1

Voyer, A. Silve, L.M. Mir, R. Scoretti and C. Poignard. Computation of the temporal evolution and spatial distribution of the electric field within a biological tissue when a pulse voltage is applied to induce electroporation. Bioelectrochemistry, in press

Azan, P.J. Caspers, T.C. Bakker Schut, S. Roy, C. Boutros, C. Mateus, E. Routier, B. Besse, D. Planchard, A. Seck, N. Kamsu Kom, G. Tomasic, S. Koljenović, V. Noordhoek Hegt, A. Celebic, M. Texier, E. Lanoy, A.M.M. Eggermont, A. Paci, C. Robert, G.J. Puppels, L.M. Mir. A Novel Spectroscopically Determined Pharmacodynamic Biomarker for Skin Toxicity in Cancer Patients Treated with Targeted Agents. Cancer Res. In press

Azan, V. Untereiner, C. Gobinet, G. D. Sockalingum, M. Breton, O. Piot and L. M. Mir. Demonstration of Protein Involvement in Living Cell Electropulsation using Confocal Raman Microspectroscopy. Sci. Rep. In press

Leguèbe, M.G. Notarangelo, M. Twarogowska, R. Natalini, C. Poignard. Mathematical model for transport of DNA plasmids from the external medium up to the nucleus by electroporation. In press in Mathematical Biosciences Journal.

A.Guittet, C. Poignard, F. Gibou. A Voronoi Interface Approach to Cell Aggregate Electropermeabilization. Accepted in Jnl Comp. Phys.

Bardet S.M., Carr L., Soueid M., Arnaud-Cormos D., Leveque P., O’Connor R.P. “Multiphoton imaging reveals that a single 10 nanosecond pulsed electric field collapses tumor perfusion in human glioblastoma xenografts”. Scientific Report, 6:34443. Published: 04 October 2016. DOI: 10.1038/srep34443

Casciola, M.; Tarek, M. A molecular insight into the electro-transfer of small molecules through electropores driven by electric fields. Biochim. Biophys. Acta. Biomembranes, 1858: 2278–2289, 2016

Pillet F; Marjanovič I; Reberšek M; Miklavčič D; Rols MP; Kotnik T. Inactivation of spores by electric arcs. Publisher: BMC Microbiol. In Press; DOI: 10.1186/s12866-016-0764-x

Phez E; Gibot L; Rols MP. How transient alterations of organelles in mammalian cells submitted to electric field may explain some aspects of gene electrotransfer process. Bioelectrochemistry, in press.

Till U; Gibot L; Mingotaud AF; Ehrhart J; Wasungu L; Mingotaud C; Souchard JP; Poinso A; Rols MP; Violleau F; Vicendo P. Drug Release by Direct Jump from Poly(ethylene-glycol-b-ε-caprolactone) Nano-Vector to Cell Membrane. Molecules, in press.

Kulbacka J; Pucek A; Kotulska M; Dubińska-Magiera M; Rossowska J; Rols MP; Wilk KA. Electroporation and lipid nanoparticles with cyanine IR-780 and flavonoids as efficient vectors to enhanced drug delivery in colon cancer. Bioelectrochemistry, DOI: 10.1016/j.bioelechem.2016.02.013

Luengo E; Martínez JM; Coustets M; Álvarez I; Teissié J; Rols MP; Raso J. A Comparative Study on the Effects of Millisecond- and Microsecond-Pulsed Electric Field Treatments on the Permeabilization and Extraction of Pigments from Chlorella vulgaris. J. Membr. Biol. DOI: 10.1007/s00232-015-9796-7

Josserand V; Kéramidas M; Lavaud J; Righini C; Vollaire J; Bellard E; Rols MP; Teissié J; Coll JL; Golzio M. Electrochemotherapy guided by intraoperative fluorescence imaging for the treatment of inoperable peritoneal micro-metastases. J Control Release Subsets: In Press; MEDLINE DOI: 10.1016/j.jconrel.2016.05.003

Pasquet L; Bellard E; Golzio M; Rols MP; Teissie J. A double-pulse approach for electrotransfection. J. Membr. Biol. DOI: 10.1007/s00232-014-9720-6

Escoffre JM; Hubert M; Teissié J; Rols MP; Favard C. Evidence for electro-induced membrane defects assessed by lateral mobility measurement of a GPi anchored protein. Eur. Biophys. J. DOI: 10.1007/s00249-014-0961-1

Rosazza C; Deschout H; Buntz A; Braeckmans K; Rols MP; Zumbusch A. Endocytosis and Endosomal Trafficking of DNA After Gene Electrotransfer In Vitro. Mol Ther Nucleic Acids DOI: 10.1038/mtna.2015.59

Rosazza C; Buntz A; Rieß T; Wöll D; Zumbusch A; Rols MP. Intracellular tracking of single-plasmid DNA particles after delivery by electroporation. Mol. Ther. DOI: 10.1038/mt.2013.182

Batista Napotnik T, Reberšek M, Vernier PT, Mali B, Miklavčič D. Effects of high voltage nanosecond electric pulses on eukaryotic cells (in vitro): A systematic review.Bioelectrochemistry 110: 1-12, 2016.

Čorović S, Mahnič-Kalamiza S, Miklavčič D. Education on electrical phenomena involved in electroporation‑based therapies and treatments: a blended learning approach. Biomed. Eng. Online 15: 36, 2016.

Dermol J, Pakhomova ON, Pakhomov AG, Miklavčič D. Cell electrosensitization exists only in certain electroporation buffers. PLOS One 11(7): e159434, 2016.

Dovgan, B, Barlič, A, Knežević, M, Miklavčič, D. Cryopreservation of Human Adipose-Derived Stem Cells in Combination with Trehalose and Reversible Electroporation. Journal of Membrane Biology: 2016.

Forjanič T., Miklavčič D. Mathematical model of tumor volume dynamics in mice treated with electrochemotherapy. Med. Biol. Eng. Comput., pp. 1–12, Sep. 2016.

Haberl Meglič S, Levičnik E, Luengo E, Raso J, Miklavčič D. The effect of temperature and bacterial growth phase on protein extraction by means of electroporation.Bioelectrochemistry 112: 77-82, 2016.

Kotnik T, Weaver JC. Abiotic gene transfer: rare or rampant? J. Membrane Biol. 249: 623-631, 2016.

Kranjc M, Bajd F, Serša I, de Boevere M, Miklavčič D. Electric field distribution in relation to cell membrane electroporation in potato tuber tissue studied by magnetic resonance techniques. Innov. Food Sci. Emerg. Technol. 37: 384-390, 2016.

Kranjc, M, Bajd, F., Serša, I., Boevere, M. de, Miklavčič, D. Electric field distribution in relation to cell membrane electroporation in potato tuber tissue studied by magnetic resonance techniques. Innovative food science & emerging technologies, ISSN 1466-8564, 2016, vol. , no. , str. 1-31

Kranjc S, Kranjc M, Ščančar J, Jelenc J, Serša G, Miklavčič D. Electrochemotherapy by pulsed electromagnetic field treatment (PEMF) in mouse melanoma B16F10 in vivo. Radiol. Oncol. 50: 39-48, 2016.

Langus, J., Kranjc, M., Kos, B., Šuštar, T., Miklavčič, D. Dynamic finite-element model for efficient modelling of electric currents in electroporated tissue. Scientific reports, ISSN 2045-2322, 2016, 6, pp. 1-11

Maček Lebar A, Velikonja A, Kramar P, Iglič A. Internal configuration and electric potential in planar negatively charged lipid head group region in contact with ionic solution.Bioelectrochemistry 111: 49-56, 2016.

Pavšelj N, Cuznar P, Veselovskyi S, Miklavčič D, Hart FX. Measuring the viscoelastic properties of tissue using changes in its capacitance: A feasibility study. Elektroteh. Vestn. 83: 99-107, 2016.

Rems L, Miklavčič D. Tutorial: Electroporation of cells in complex materials and tissue. J. Appl. Phys. 119: 201101, 2016.

Sharabi S, Kos B, Last D, Guez D, Daniels D, Harnof S, Mardor Y, Miklavčič D. A statistical model describing combined irreversible electroporation and electroporation-induced blood-brain barrier disruption. Radiol. Oncol. 50: 28-38, 2016.

Stepišnik T, Jarm T, Grošelj A, Edhemović I, Đokić M, Ivanecz A, Trotovšek B, Brecelj E, Potrč S, Čemažar M, Glumac N, Pečnik T, Verberič B, Gadžijev E, Strojan P, Snoj M, Miklavčič D, Serša G .Elektrokemoterapija - učinkovita metoda zdravljenja tumorjev s kombinacijo kemoterapevtika in električnega polja. Zdrav. Vestn. 85: 41-55, 2016.

Sweeney DC, Reberšek M, Dermol J, Rems L, Miklavčič D, Davalos RV. Quantification of cell membrane permeability induced by monopolar and high-frequency bipolar bursts of electrical pulses. Biochim. Biophys. Acta 1858: 2689-2698, 2016.

Velikonja A, Kramar P, Miklavčič D, Maček Lebar A. Specific electrical capacitance and voltage breakdown as a function of temperature for different planar lipid bilayers. Bioelectrochemistry 112: 132-137, 2016.

Zorec, B., Škrabelj, D., Marinček, M., Miklavčič, D., Pavšelj, N. The effect of pulse duration, power and energy of fractional Er:YAG laser for transdermal delivery of differently sized FITC dextrans. International journal of pharmaceutics, ISSN 0378-5173. [Print ed.], Jan. 2017, vol. 516, no. 1/2, pp. 204-213, http://www.sciencedirect.com/science/article/pii/S0378517316310262, doi: 10.1016/j.ijpharm.2016.10.060.

GROŠELJ, A, KRŽAN, M, KOSJEK, T, BOŠNJAK, M, SERŠA, G, ČEMAŽAR, M. Bleomycin pharmacokinetics of bolus bleomycin dose in elderly cancer patients treated with electrochemotherapy. Cancer chemotherapy and pharmacology, 2016, vol. 77, 939-947, doi: 10.1007/s00280-016-3004-z.

ŠTIMAC, M, KAMENŠEK, U, ČEMAŽAR, M, KRANJC, S, CÖR, A, SERŠA, G. Tumor radiosensitization by gene therapy against endoglin. Cancer gene therapy, 2016, doi: 10.1038/cgt.2016.20.

DOLINŠEK, T, SERŠA, G, PROSEN, L, BOŠNJAK, M, ŠTIMAC, M, RAZBORŠEK, U, ČEMAŽAR, M. Electrotransfer of plasmid DNA encoding an anti-mouse endoglin (CD105) shRNA to B16 melanoma tumors with low and high metastatic potential results in pronounced anti-tumor effects. Cancers, doi: 10.3390/cancers8010003.

BERTINO, G, SERŠA, G, DE TERLIZZI, F, OCCHINI, A, PLASCHKE, CC, GROŠELJ, A, LANGDON, C, GRAU, JJ., MCCAUL, JA., HEUVELING, D, ČEMAŽAR, M, STROJAN, P, DE BREE, R, LEEMANS, C. R, WESSEL, I, GEHL, J, BENAZZO, M. European research on electrochemotherapy in head and neck cancer (EURECA) project : results of the treatment of skin cancer. European Journal of Cancer, 2016, 63, 41-52, doi: 10.1016/j.ejca.2016.05.001.

LAMBRICHT, L, LOPES, A, KOS, Š, SERŠA, G, PRÉAT, V, VANDERMEULEN, G. Clinical potential of electroporation for gene therapy and DNA vaccine delivery. Expert opinion on drug delivery, 2016, vol. 13, 295-310, doi: 10.1517/17425247.2016.1121990.

GRABNER, S, MODEC, B, BUKOVEC, N, BUKOVEC, P, ČEMAŽAR, M, KRANJC, S, SERŠA, G, ŠČANČAR, J. Cytotoxic trans-platinum(II) complex with 3-hydroxymethylpyridine : synthesis, x-ray structure and biological activity evaluation. Journal of inorganic biochemistry, doi: 10.1016/j.jinorgbio.2016.04.031.

PREVC, A, BEDINA ZAVEC, A, ČEMAŽAR, M, KLOBOVES-PREVODNIK, V, ŠTIMAC, M, TODOROVIĆ, V, STROJAN, P, SERŠA, G. Bystander effect induced by electroporation is possibly mediated by microvesicles and dependent on pulse amplitude, repetition frequency and cell type. The journal of membrane biology, 2016, doi: 10.1007/s00232-016-9915-0.

KOS, Š, BLAGUS, T, ČEMAŽAR, M, LAMPREHT TRATAR, U, ŠTIMAC, M, PROSEN, L, DOLINŠEK, T, KAMENŠEK, U, KRANJC, S, STEINSTRAESSER, L, VANDERMEULEN, G, PRÉAT, V, SERŠA, G. Electrotransfer parameters as a tool for controlled and targeted gene expression in skin. Molecular therapy, Nucleic acids, 2016, 5, doi: 10.1038/mtna.2016.65.

PROSEN, L, HUDOKLIN, S, ČEMAŽAR, M, ŠTIMAC, M, LAMPREHT TRATAR, U, OTA, M, ŠČANČAR, J, ROMIH, R, SERŠA, G. Magnetic field contributes to the cellular uptake for effective therapy with magnetofection using plasmid DNA encoding against Mcam in B16F10 melanoma in vivo. Nanomedicine, 2016, 11, 627-641, doi: 10.2217/nnm.16.4.

HRIBERNIK, A, ČEMAŽAR, M, SERŠA, G, BOŠNJAK, M, SNOJ, M. Effectiveness of electrochemotherapy after IFN-[alpha] adjuvant therapy of melanoma patients. Radiology and oncology, 2016, 50, 21-27, doi: 10.1515/raon-2015-0048.

KRANJC, S, KRANJC, M, ŠČANČAR, J, JELENC, J, SERŠA, G, MIKLAVČIČ, D. Electrochemotherapy by pulsed electromagnetic field treatment (PEMF) in mouse melanoma B16F10 in vivo. Radiology and oncology, 2016, 50, 39-48, doi: 10.1515/raon-2016-0014.

DOLINŠEK, T, PROSEN, L, ČEMAŽAR, M, POTOČNIK, T, SERŠA, G. Electrochemotherapy with bleomycin is effective in BRAF mutated melanoma cells and interacts with BRAF inhibitors. Radiology and oncology, 2016, 50, 274-279, doi: 10.1515/raon-2016-0042.

ČEMAŽAR, M, AMBROŽIČ, J, PAVLIN, D, SERŠA, G, POLI, A, KRHAČ LEVAČIĆ, A, TEŠIĆ, N, LAMPREHT TRATAR, U, RAK, M, TOZON, N. Efficacy and safety of electrochemotherapy combined with peritumoral IL-12 gene electrotransfer of canine mast cell tumours. Veterinary and comparative oncology, 2016, doi: 10.1111/vco.12208.

TOZON, N, KRAMARIČ, P, KADUNC KOS, V, SERŠA, G, ČEMAŽAR, M. Electrochemotherapy as a single or adjuvant treatment to surgery of cutaneous sarcoid tumours in horses: a 31-case retrospective study. Veterinary record, 2016, doi: 10.1136/vr.103867.

ŽNIDAR, K, BOŠNJAK, M, ČEMAŽAR, M, HELLER, L C. Cytosolic DNA sensor upregulation accompanies DNA electrotransfer in B16.F10 melanoma cells. Molecular therapy, Nucleic acids, 2016, 5, doi: 10.1038/mtna.2016.34.


Recent publications

Pillet F, Marjanovič I, Reberšek M, Miklavčič D, Rols MP, Kotnik T. Inactivation of spores by electric arcs. BMC Microbiol. 16: 148, 2016.

Rosazza C, Haberl Meglič S, Zumbusch A, Rols MP, Miklavčič D. Gene electrotransfer: a mechanistic perspective. Curr. Gene Ther. 16: 98, 2016.

Kamensek U, Rols M-P, Čemazar M, Golzio M. Visualization of nonspecific antitumor effectiveness and vascular effects of gene electro-transfer to tumors. Curr. Gene Ther. 16: 90, 2016.

Y. Calvet and L. M. Mir. The promising alliance of anticancer electrochemotherapy with immunotherapy. Cancer Metastases Reviews, 35: 165, 2016.

Casciola, M.; Tarek, M. A molecular insight into the electro-transfer of small molecules through electropores driven by electric fields. Biochim. Biophys. Acta. Biomembranes, 1858: 2278, 2016.

Stimac, M, Kamensek, U, Cemazar, M, Kranjc, S, Cor, A, Sersa, G. Tumor radiosensitization by gene therapy against endoglin. Curr. Gene Ther.  23: 214, 2016.


24thInternational Symposium on Bioelectrochemistryand Bioenergetics of the BioelectrochemicalSociety
Lyon, July 3 –7, 2017

2ndWorld Congress on Electroporation and Pulsed Electric Fields
Norfolk, September 24 –28, 2017



- Dr Lluis M. MIR, for CNRS, France,

- Pr Damijan MIKLAVCIC, for UL, Slovenia.